Nucleic Acid Controls
EPICYPHER INC BRG1/SMARCA4 CUTANA™ CUT&RUN Antibody, 100 µL
This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated CUTANA approach using EpiCypher optimized protocols and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. BRG1 antibody produces CUT&RUN peaks primarily flanking transcription start sites (TSSs, Figure 1). BRG1 peaks show a large degree of overlap with BRD4 peaks (Figure 2), as has been reported in the literature [Conrad et al., 2017].
Abcam Pyronin Y (Pyronin G), Cationic dye (RNA), 5G
MW 302.8 g/mol. Cationic dye that intercalates RNA and is visible as a red band during electrophoresis. Shown to accumulate in the mitochondria of viable cells. At low concentrations the cytostatic effect is cell arrest in the G1 phase, whereas at high concentrations the cytotoxic effect is cell arrest in the G2 and S phase. Used in combination with Alcian Blue to assess and study pulmonary diseases, and in combination with Methyl Green to stain nucleic acids in paraffin sections. Additionally, has been used to measure RNA in CHO cells by flow cytometry.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Q x -314 chloride (N-Ethyllidocaine chloride) (mM/ml), Na+ channel blocker, 100MM_ML
MW 298.8 Da, Purity >99%. A membrane impermeable quaternary lidocaine derivative. Blocks voltage-sensitive Na+ channels. Soluble in 1 ml water to give specified mM/ml concentration.
The product is subject to the following: Abcam Restricted Use Statement
Thomas Scientific DirectAmpT Tissue Genomic DNA Amplification Kit 100 reactio
DirectAmpT Tissue Genomic DNA Amplification Kit 100 reactio
Zymo Research Corporation OneStep PLUS qMethyl PCR Kit
PCR Kit No bisulfite conversion needed from DNA sample to results in just one step. Accurate quantification of DNA methylation levels at the genomic regions of choice. Ideal for rapid screening of DNA methylation at single and multi-locus. 1/EA